Medical Genome Facility - Florida

The Mayo Clinic Medical Genome Facility (MGF) in Florida provides investigators easy access to state-of-the-art DNA and RNA services. This centralized core laboratory offers expert consultation with experimental design and cost effective, rapid generation of top-quality data. We provide a wide range of services, including comprehensive expression profiling using NanoString nCounter technology, Illumina MiSeq next-generation target sequencing and large-scale genotyping with the Agena Bioscience MassARRAY system.

Areas of focus

Gene Expression

NanoString nCounter provides a sensitive, highly reproducible, multiplexed method for detecting a broad range of expression levels.

The nCounter assay labels mRNA with target-specific color-coded probes without the use of reverse transcription or amplification. This allows for direct analysis of a wide variety of samples, including total RNA, cell or tissue lysates, RNA extracted from formalin-fixed paraffin embedded tissues and blood.

NanoString applications:

  • Gene expression (up to 800 genes)
  • miRNA expression
  • lncRNA
  • Copy number variations (CNV)

Next-Generation Sequencing

Next-generation sequencing (NGS) gives clinicians and researchers detailed information about the genetic makeup of their samples, allowing them to identify and characterize the role of genetics in disease and treatment. The facility offers several NGS services with the Illumina MiSeq, including:

  • Library preparation
  • Targeted sequencing
  • Small genome sequencing
  • RNA-seq
  • Regulation (ChIP-Seq)
  • 16S metagenomics

Additional NGS equipment includes: Qubit Fluorometer, Agilent Bioanalyzer and Covaris DNA shearer.

Genotyping

Highly scalable, customizable and affordable, the Agena Bioscience MassARRAY System, a mass spectrometry-based system, provides mutation profiling or SNP genotyping with greater sensitivity than NGS.

Contact

For more information, please contact:
Jennifer Gass, Ph.D.
Phone: 904-953-6471