Tissue and Cell Molecular Analysis (TACMA) Laser Capture Microdissection (LCM)
Using an enclosed microscope and a software interface, investigators are able to isolate individual cells or clusters of cells from lightly fixed cryosections or paraffin sections mounted on either membrane or glass slides. The current Veritas LCM machine is capable of handling both normal brightfield and fluorescence microscopy. Furthermore, images can be generated during the procedure for use in later documentation.
Following the procedure, investigators have the option of extracting protein, RNA, or DNA from their captured cells. Alternatively, they may submit their microsamples to the Biospecimens Accessioning and Process Shared Resource for subsequent RNA extraction.
TACMA provides training and information to research individuals wishing to use the Veritas system, and staff members are available in the event of problems or questions. TACMA personnel can also perform the LCM procedure upon special request, provided one or more persons familiar with the particular tissues to be studied are available to point out the appropriate cellular areas to target for capture.
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