Electron Microscopy Shared Resource

Jeffrey Salisbury, Ph.D.
Director

The Electron Microscopy Core Facility (EMCF) is a research resource laboratory providing specimen preparation, microscopy and digital imaging services to investigators from both clinical and basic science laboratories within Mayo Clinic. The facility, which is a Shared Resource of Mayo Clinic Cancer Center as well, is fully equipped to offer standard transmission (TEM) and scanning (SEM) electron microscopy. The facility also performs X-ray probe microanalysis and immuno-gold labeling procedures.

Facilities, Equipment and Staffing

The EMCF is staffed by 14 technicians available to provide a number of services including:

• Tissue Fixation and Embedding
• Digital imaging
• Ultrastructural pathology
• Immunoelectron microscopy
• X-Ray microanalysis
• Scanning electron microscopy
• Transmission Electron Microscopy

Jon Charlesworth
Manager

Major equipment include:

Philips CM10 Transmission Electron Microscope - for both diagnostic and research samples and features the Biotwin lens configured for high contrast and low magnification imaging. A Gatan digital camera has been added to this microscope

Philips Technai T12 Transmission Electron Microscope - used for diagnostic, research and analytical samples. In addition to the standard transmission mode this instrument has an X-ray microanalysis system and fitted with a 2k x 2k digital camera system.

JEOL 1400 Transmission Electron Microscope -for high-resolution biomedical research samples and is the workhorse for research specimens. Two Gatan digital cameras have been added to this microscope.

Hitachi S4700 Field Emission Scanning Electron Microscope - completely digital, high resolution SEM that is equipped with secondary, backscatter, cathode luminescence and EDS detectors.

Specimen preparation equipment include:

• RMC tissue processors
• Pelco biowave microwave tissue processor
• Ultramicrotomes
• Critical point dryer
• High vacuum evaporator
• Sputter coaters
• Duo ion beam coater
• Freeze substitution system