TRANSMISSION ELECTRON MICROSCOPY

Specimen Preparation

• Standard tissue preparation from initial fixation through embedding
• Microwave-accelerated processing is available

Shadow Casting

• A Denton model DV502A is used to rotary shadow samples with aluminum, tungsten or platinum.

Negative Staining

• Negative staining is available using phosphotungstic acid, uranyl acetate or ammonium molybdate

Thin Sectioning

• Plastic embedded tissue can be sectioned for the electron microscope with thickness ranges from 60nm to 250nm

Thick Sectioning

• Plastic embedded tissue can be sectioned for the light microscope with thickness ranges from 0.25µ to 2.0µ
• Standard stain used is toluidine blue
• Standard stains used are uranyl acetate and lead citrate