TECHNOLOGIES USED IN THE LABORATORY- Lipid biochemical and analytical methods
- Chemical synthesis of radioactive and fluorescent lipid analogs
- Quantitative fluorescence microscopy of living cells
- Laser scanning confocal microscopy
- Immunofluorescence-staining of cells
- Microinjection of antibodies, DNA, and fluorescent probes into cells
- Tissue culture of various cell types, including human fibroblasts and rat lung epithelial cells
- Protein isolation-biochemistry
- Antibody production/purification
- SDS-PAGE one and two-dimensional protein gel electrophoresis
- Gene cloning, sequencing, PCR
- Northern, Western, Southern blot analysis
- Protein overexpression systems
- Cell transfection/expression of tagged proteins in living cells
- Computer graphics
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