ProteinSimple's NanoPro 1000, the nanofluidic immunoassay platform used in the Proteomics Core, employs separation of native proteins by isoelectric focusing (IEF) in small capillaries (400 nanoliter volumes). Once focusing is complete, proteins are cross-linked to the walls of the capillaries and immunoassay reagents — such as primary, horseradish peroxidase (HRP)-labeled secondary antibody or hydrogen peroxide-luminol — are introduced.
This assay is very sensitive to low-abundance antigens, and because proteins are separated based on charge, phosphoproteins are resolved from nonphosphorylated forms, allowing quantitation of peak areas of the different isoforms.
Assays for several major cell signaling proteins and their phosphorylated forms are available, including ERK1/2 (MAPK), Akt, EGFR, MEK1 and MEK2. All NanoPro assay projects must be reviewed and approved by the Proteomics Core.
Project costs are on a per-capillary basis, along with applicable technician time for sample preparation and analysis.
For more information or to initiate a service or project request, email the Proteomics Core.
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