Protein Identification and Characterization
Protein identification is a central mass spectrometry-based service offered by the Proteomics Core. This service is most frequently, but not exclusively, performed on stained SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gel bands or spots.
Protein characterization requires the identification and location of post-translational modifications, detection of mutations or truncations, and intact mass molecular weight determination. Requests for protein characterization should not be considered routine, but rather more experimental because the desired outcome is sample-dependent and there is a need for additional personnel effort, instrument time and interactive data analysis.
Before submitting samples, all first-time protein identification and characterization clients must contact the Proteomics Core. The details required for successful analysis are sample dependent and heavily dependent on proper sample handling, so interested users must clearly understand these requirements before submitting samples.
Protein identification costs are on a per-band or per-spot basis. Protein characterization includes the protein identification costs, along with additional costs for data and sample analysis using alternative and supportive techniques.
For more information or to initiate a service or project request, email the Proteomics Core.
© 2013 Mayo Foundation for Medical Education and Research. All rights reserved.