Receptor Biology - Steven M. Sine

Publications

Peer-reviewed publications

  1. Sine, S.M., T. E. Conklin and W. H. Okamura. Studies on Vitamin D and its analogs: 3-Deoxy-A-Homovitamin D3, a model synthesis. Journal of Organic Chemistry 39: 3797-3799, 1974.
  2. Wing, R. M., W. H. Okamura, M. R. Pirio, S. M. Sine and A. W. Norman. Vitamin D in solution: Conformations of vitamin D3, 1, 25-Dihydroxy-vitamin D3, and Dihydrotachysterol. Science 186: 939-941, 1974.
  3. Sine, S.M. and P. Taylor. Functional consequences of agonist-mediated state transitions in the cholinergic receptor. Journal of Biological Chemistry 254: 3315-3325, 1979.
  4. Sine, S.M. and P. Taylor. Relationship between agonist occupation and permeability response of the cholinergic receptor revealed by bound cobra-toxin. Journal of Biological Chemistry 255: 10144-10156, 1980.
  5. Sine, S.M. & P. Taylor. Relationships between reversible antagonist occupancy and the functional capacity of the acetylcholine receptor. Journal of Biological Chemistry 256: 6692-6699, 1981.
  6. Sine, S.M. & P. Taylor. Local anesthetics and histrionicotoxin are allosteric inhibitors of the acetylcholine receptor. Journal of Biological Chemistry 257: 8106-8114, 1982.
  7. Sine, S.M. & J. H. Steinbach. Agonists block currents through acetylcholine receptor channels. Biophysical Journal 46: 277-284, 1984.
  8. Sine, S.M. & J. H. Steinbach. Activation of a nicotinic acetylcholine receptor. Biophysical Journal 45: 175-185, 1984.
  9. Sine, S.M. & J. H. Steinbach. Acetylcholine receptor activation by a site-selective ligand: Nature of brief open and closed states in BC3H-1 cells. Journal of Physiology 370: 357-379, 1985.
  10. Sine, S.M. & J. H. Steinbach. Activation of acetylcholine receptors by low concentrations of agonist. Journal of Physiology 373: 129-162, 1986.
  11. Sine, S.M. & J. H. Steinbach. Activation of acetylcholine receptors by high concentrations of agonist. Journal of Physiology 385: 325-359, 1987.
  12. Sigworth, F. & S. M. Sine. Data transformations for improved display and fitting of single-channel dwell time histograms. Biophysical Journal 52: 1047-1054, 1987.
  13. Claudio, T., W. N. Green, D. S. Hartman, D. Hayden, H. L. Paulson, F. J. Sigworth, S. M. Sine and A. Swedlund. Genetic reconstitution of functional acetylcholine receptor channels in mouse fibroblasts. Science 238: 1688-1694, 1987.
  14. Sine, S.M. Functional properties of human skeletal muscle acetylcholine receptors expressed by the TE671 cell line. Journal of Biological Chemistry 263: 18052-18062, 1988.
  15. Sine, S.M., T. Claudio & F. J. Sigworth. Activation of Torpedo acetylcholine receptors expressed in mouse fibroblasts: Single channel current kinetics reveal distinct agonist binding affinities. Journal of General Physiology 96: 395-437, 1990.
  16. Sine, S.M. & T. Claudio. Stable expression of the mouse nicotinic acetylcholine receptor in fibroblasts: Comparison of receptors in native and transfected cells. Journal of Biological Chemistry 266: 13679-13689, 1991.
  17. Sine, S.M. & T. Claudio. Gamma- and delta-subunits regulate the affinity and cooperativity of ligand binding to the acetylcholine receptor. Journal of Biological Chemistry 266: 19369-19377, 1991.
  18. Sine, S.M. Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that determine curare selectivity. Proceedings of the National Academy of Sciences 90: 9436-9440, 1993.
  19. Sine, S.M., P. Quiram, F. Papanikolaou, H.-J. Kreienkamp & P. Taylor. Conserved tyrosines in the a subunit of the nicotinic acetylcholine receptor stabilize quaternary ammonium groups of agonists and curariform antagonists. The Journal of Biological Chemistry 269: 8808-8816, 1994.
  20. Krienkamp, H., Sine, S.M., Maeda, R., & P. Taylor. Glycosylation sites selectively interfere with a-toxin binding to the nicotinic acetylcholine receptor. Journal of Biological Chemistry 269: 8108-8114, 1994.
  21. Bouzat, C., N. Bren & S. M. Sine. Structural basis of the different gating kinetics of fetal and adult acetylcholine receptors. Neuron 13: 1395-1402, 1994.
  22. Fu, D.-X. & S. M. Sine. Competitive antagonists bridge the a-g subunit interface of the acetylcholine receptor through quaternary ammonium-aromatic interactions. Journal of Biological Chemistry 269: 26152-26157, 1994.
  23. Ohno, K., D. O. Hutchinson, M. Milone, J. M. Brengman, C. Bouzat, S. M. Sine & A. G. Engel. Myasthenic syndrome caused by a mutation in the M2 domain of the acetylcholine receptor e subunit. Proceedings of the National Academy of Sciences 92: 758-762, 1995.
  24. Chen, J., Y. Zhang, G. Akk, S. Sine & A. Auerbach. Activation kinetics of recombinant mouse nicotinic acetylcholine receptors with mutations at a subunit residue tyrosine 190. Biophysical Journal 69: 849-859, 1995.
  25. Kreienkamp, H.-J., R. K. Maeda, S. M. Sine & P. Taylor. Intersubunit contacts governing assembly of the mammalian nicotinic acetylcholine receptor. Neuron 14: 635-644, 1995.
  26. Sine, S M., H.-J. Kreienkamp, N. Bren, R. Maeda & P. Taylor. Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of determinants of a-Conotoxin M1 selectivity. Neuron 15: 205-211, 1995.
  27. Sine, S.M., K. Ohno, C. Bouzat, A. Auerbach, M. Milone, J. N. Pruitt & A. G. Engel. Mutation of the acetylcholine receptor a-subunit causes a slow-channel myasthenic syndrome by enhancing agonist binding affinity. Neuron 15: 229-239, 1995.
  28. Ohno, K., H.-L. Wang, M. Milone, N. Bren, J. M. Brengman, S. Nakano, P. Quiram, J. N. Pruitt, S. M. Sine & E. G. Engel. Congential myasthenic syndrome caused by decreased agonist binding affinity due to a mutation in the acetylcholine receptor e subunit. Neuron 17: 157-170, 1996.
  29. Engel, A. G., K. Ohno, M. Milone, H.-L. Wang, S. Nakano, C. Bouzat, J. N. Pruitt II, D. O. Hutchinson, J. M. Brengman, N. Bren, J. P. Sieb & S. M. Sine: New mutations in acetylcholine receptor subunit genes reveal heterogeneity in the slow-channel congenital myasthenic syndrome. Human Molecular Genetics 5: 1217-1227, 1996.
  30. Akk, G., S. Sine & A. Auerbach. Binding sites contribute unequally to the gating of mouse nicotinicaD200N acetylcholine receptors. Journal of Physiology 496: 185-196, 1996.
  31. Prince, R. J. & S. M. Sine. Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that determine agonist selectivity. Journal of Biological Chemistry 271: 25770-25777, 1996.
  32. Fu, D.-X. & S. M. Sine: Asymmetric contribution of the conserved disulfide loop to subunit oligomerization and assembly of the nicotinic acetylcholine receptor. Journal of Biological Chemistry 271: 31479-31484, 1996.
  33. Engel, A. G., K. Ohno, C. Bouzat, S. M. Sine & R. C. Griggs. End-plate acetylcholine receptor deficiency due to nonsense mutations in the e subunit. Annals of Neurology 40: 810-817, 1996.
  34. Ohno, K., P. Quiram, M. Milone, H.-L. Wang, M. Harper, J. Pruitt, J. Brengman, L. Pao, K. Fischbeck, T. Crawford, S. Sine & A. Engel. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: identification and functional characterization of six new mutations. Human Molecular Genetics 6: 753-766, 1997.
  35. Wang, H-L., A. Auerbach, N. Bren, K. Ohno, A. Engel & S. Sine. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases the rate of agonist dissociation. Journal of General Physiology 109: 757-766, 1997.
  36. Tsigelny, I., N. Sugiyama, S. Sine & P. Taylor. A model of the nicotinic receptor extracellular domain based on sequence identity and residue location. Biophysical Journal 73: 52-66, 1997.
  37. Milone, M., H.-L.Wang, K. Ohno, T. Fukudome, N. Pruitt, N. Bren, S. Sine & A. Engel. Slow channel myasthenic syndrome caused by enhanced activation, desensitization, and agonist binding affinity due to mutation in the M2 domain of the acetylcholine receptor a subunit. Journal of Neuroscience 17: 5651-5665, 1997.
  38. Sine, S. Identification of equivalent residues in the g, d, and e subunits of the nicotinic receptor that contribute to a-bungarotoxin binding. Journal of Biological Chemistry 272: 23521-23527, 1997.
  39. Bren, N. & S. Sine. Identification of residues in the adult nicotinic acetylcholine receptor that confer selectivity for curariform antagonists. Journal of Biological Chemistry 272: 30793-30798, 1997.
  40. Sugiyama, N., P. Marchot, C. Kawanishi, H. Osaka, B. Molles, S. Sine & P. Taylor. Residues at the subunit interfaces of the nicotinic acetylcholine receptor that contribute to
    a-conotoxin M1 binding. Molecular Pharmacology 53: 787-794, 1998.
  41. Wang, H-L., M. Milone, K. Ohno, R. Prince, T. Fukudome, X.-M. Shen, J. Brengman, R. Griggs, S. M. Sine & A. Engel. Mode switching kinetics by a naturally occurring mutation in the cytoplasmic loop of the human acetylcholine receptor e subunit. Neuron 20: 575-588, 1998.
  42. Prince, R. & S. Sine. Epibatidine binds with unique site- and state-selectivity to muscle nicotinic acetylcholine receptors. Journal of Biological Chemistry 273: 7843-7849, 1998.
  43. Quiram, P. & S. Sine. Identification of residues in the neuronal a7 receptor that confer selectivity for conotoxin ImI. Journal of Biological Chemistry 273: 11001-11006, 1998.
  44. Quiram, P. & S. Sine. Structural elements in a-conotoxin ImI essential for binding to neuronal a7 receptors. Journal of Biological Chemistry 273: 11007-11011, 1998.
  45. Prince, R. & S. Sine. Epibatidine activates muscle acetylcholine receptors with unique site-selectivity. Biophysical Journal 75: 1817-1827, 1998.
  46. Wang, H.-L., M. Milone, K. Ohno, X.-M. Shen, A. Tsujuno, A. Paola, P. Tonali, J. Brengman, A. G. Engel & Sine, S.M. Acetylcholine receptor M3 domain: Stereochemical and volume contributions to channel gating. Nature Neuroscience 2: 226-233, 1999.
  47. Osaka, H., S. Malany, J. Kanter, S. M. Sine & P. Taylor. Subunit interface selectivity of the a-neurotoxins for the nicotinic acetylcholine receptor. Journal of Biological Chemistry 274: 9581-9586, 1999.
  48. Quiram, P., J. Jones & S. M. Sine. Identification of Pairs of Interacting Residues Between Neuronal a7 Receptors and a-Conotoxin ImI. Journal of Biological Chemistry 274: 19517-19525, 1999.
  49. Prince, R. J. & S. M. Sine. Acetylcholine and epibatidine binding to muscle acetylcholine receptors distinguish between concerted and uncoupled models. Journal of Biological Chemistry 274: 19623-19629, 1999.
  50. Quiram, P., K. Ohno, M. Milone, M. Patterson, N. Pruitt, J. Brengman, S. Sine & A. Engel. Mutation causing congenital myasthenia reveals acetylcholine receptor b/d subunit interaction essential for assembly. Journal of Clinical Investigation 104: 1403-1410, 1999.
  51. Quiram, P., M. McIntosh & S. Sine. Pairwise interactions between neuronal a7 receptors and a-conotoxin PnIB. Journal of Biological Chemistry 275: 4889-4996, 2000.
  52. Osaka, H., S. Malany, B. E. Molles, S. M. Sine & P. Taylor. Pairwise electrostatic interactions between alpha-neurotoxins and gamma, delta and epsilon subunits of the nicotinic acetylcholine receptor. Journal of Biological Chemistry 275: 5478-5484, 2000.
  53. Bren, N. & S. M. Sine. Hydrophobic pairwise interactions stabilize a-conotoxin MI in the muscle acetylcholine receptor binding site. Journal of Biological Chemistry 275: 12692-12700, 2000.
  54. Bouzat, C., F. Barrantes & S. Sine. Nicotinic receptor fourth transmembrane domain: hydrogen bonding by conserved threonine contributes to channel gating kinetics. Journal of General Physiology 115: 663-671, 2000.
  55. Grosman, C., F. Salamone, S. M. Sine & A. Auerbach. The extracellular linker of muscle acetylcholine receptors is a gating control element. Journal of General Physiology 116, 327-339, 2000.
  56. Wang, H. L, K. Ohno, M. Milone, J. Brengman, A. Evoli, A. P. Batocchi, L. Middleton, K. Christodoulou, A. G. Engel & S. M. Sine. Fundamental gating mechanism of nicotinic receptor channel gating revealed by mutation causing a congenital myasthenic syndrome. Journal of General Physiology 116: 449-460, 2000.
  57. Malany, S., H. Osaka, S. M. Sine & P. Taylor. The orientation of a-neurotoxin at the subunit interfaces of the nicotinic acetylcholine receptor. Biochemistry 39: 15388-15398, 2000.
  58. Iba¯ez-Tallon, I., J. Miwa, H.-L. Wang, N. Adams, G. Crabtree, S. M. Sine & N. Heintz. Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1. Neuron 33, 893-903, 2002.
  59. Bouzat, C., F. Gumilar, M. Esandi & S. M. Sine. Subunit-selective contribution to channel gating of the M4 domain of the nicotinic receptor. Biophysical Journal 82, 1920-1929, 2002.
  60. Molles, B. E., Rezai, P., Kline, E., J. McArdle, S. M. Sine & P. Taylor. Identification of residues at the a and e subunit interfaces mediating species selectivity of Waglerin-1 for nicotinic acetylcholine receptors. Journal of Biological Chemistry 277, 5433-5440, 2002.
  61. Molles, B. E., Tsigelny, I., Nguyen, P. D., Gao, S. X., Sine, S.M. & P. Taylor. Residues in the epsilon subunit of the nicotinic acetylcholine receptor interact to confer selectivity of waglerin-1 for the alpha-epsilon subunit interface site. Biochemistry. 41:7895-7906, 2002.
  62. Sine. S. M., H.-L. Wang & N. Bren. Lysine scanning mutagenesis delineates structure of nicotinic receptor ligand binding domain. Journal of Biological Chemistry 277, 29210-29223, 2002.
  63. Prince, R. J., Pennington, R. & S. M. Sine. Mechanism of tacrine block at adult human muscle nicotinic acetylcholine receptors. Journal of General Physiology 120, 369-393, 2002.
  64. Sine, S.M., X.-M. Shen, H.-L. Wang, J. Brengmann, W. Y. Lee, N. Bren & A. G. Engel Naturally-occurring mutations at the acetylcholine receptor binding site independently alter ACh binding and channel gating. Journal of General Physiology 120, 483-496, 2002.
  65. Shen, X.-M., Ohno, K., Tsujino, A., Brengman, J., Gingold, M., Sine, S.M. & Engel, A. G. Mutation causing severe myasthenia reveals functional asymmetry of AChR signature cys-loops in agonist binding and gating. Journal of Clinical Investigation 111, 497-505, 2003.
  66. Curariform Antagonists Bind in Different Orientations to Acetylcholine Binding Protein. Fan Gao, Nina Bren, Alicia Little, Hai-Long Wang, Scott B. Hansen, Todd T. Talley, Palmer Taylor & Steven M. Sine. Journal of Biological Chemistry 278, 23020-23026, 2003.
  67. Curariform Antagonists Bind in Different Orientations to the Nicotinic Receptor Ligand Binding Domain. Hai-Long Wang, Fan Gao, Nina Bren & Steven M. Sine. Journal of Biological Chemistry 278, 32284-32291, 2003.
  68. Asymmetric structural motions of the homomeric a7 nicotinic receptor ligand binding domain revealed by molecular dynamics simulation. Henchman, R. H., Wang, H-L., Sine, S.M., Taylor, P. & McCammon, J. A. Biophysical Journal 85, 3007-3018, 2003.
  69. Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that contribute to epibatidine selectivity in the desensitized state. Penninton, R., Gao, F., Sine, S.M. & Prince, R. J. (submitted to JBC)

Review articles

  1. Taylor, P., G. Weiland, S. M. Sine, C. Chignell & R. Brown. Cholinergic receptor state transitions and local anesthetic action. In: Progress in Anesthesiology, Vol. 2, 1980, pp. 175-188.
  2. Taylor, P & S. M. Sine. The relationship between ligand occupation and activation of the nicotinic cholinergic receptor: In: Stjarne, L., P. Hedqvist, H. Lagercrantz and A. Wennmalm: Chemical Neurotransmission: 75 Years. Second Nobel Conference, Academic Press, London, 1983, pp. 347-359.
  3. Sine, S.M. & P. Taylor. Ligand occupation and the functional states of the nicotinic cholinergic receptor. In: Towards the Understanding of Receptors. J. Lamble, Ed., Elsiever Press, Vol. 2, 1982, pp. 125-133.
  4. Taylor, P. & S. M. Sine. Ligand occupation and the functional states of the nicotinic cholinergic receptor. Trends in Pharmacological Science 3: 197-200, 1982.
  5. Taylor, P., S. Sine, P. Culver & D. Johnson. The coupling between ligand occupation and response of the nicotinic acetylcholine receptor: Correlations with receptor structure. In: Singer, T., T. Mansour & R. Ondarza (Eds.): Mechanism of Drug Action. Academic Press, 1983, pp. 9-26.
  6. Sine, S.M. & P. Taylor. Studies of ligand occupancy and permeability response of acetylcholine receptors on intact cells. In: Reid, E., G. M. Cook and D. J. Moore (Eds.): Investigations of membrane-located receptors. Methodological Surveys in Biochemistry and Analysis. Plenum Press, Vol. 13, 1984, pp. 369-380.
  7. Steinbach, J. H., M. Covarrubias, S. Sine & J. Steele. Function of mammalian nicotinic acetylcholine receptors. In: Maelicke, A. (Ed.): The Nicotinic Acetylcholine Receptor: Structure and Function. Springer-Verlag, 1987, pp. 219-232.
  8. Sine, S.M. & J. H. Steinbach. Function of mammalian nicotinic acetylcholine receptors: Agonist concentration dependence of single channel current kinetics. In: Hoffman, J. and G. Giebisch (Eds.): Current Topics in Membranes and Transport: Molecular Biology of Ion Channels. Academic Press, Vol. 33, 1988, pp. 134-145.
  9. Prince, R. & S. M. Sine. The ligand binding domains of the nicotinic acetylcholine receptor. In: F. Barrantes (Ed.): Neurosciences Intelligence Series: The acetylcholine receptor: current views and future trends. Landes Bioscience, Austin TX, pp. 31-59.
  10. Engel, A. G., K. Ohno, M. Milone, & S. M. Sine. Congenital myasthenic syndromes caused by mutations in acetylcholine receptor genes. Neurology 48 (Suppl 5): S28-S34, 1997.
  11. Engel., A., K. Ohno, M. Milone & S. M. Sine. Congenital myasthenic syndromes: new insights from molecular genetic and patch clamp studies. Annals of the New York Academy of Sciences 841: 140-156, 1997.
  12. Sine, S.M., N. Bren & P. Quiram. Molecular dissection of subunit interfaces in the nicotinic acetylcholine receptor. Journal of Physiology (Paris) 92: 101-105, 1998.
  13. Taylor, P., H. Osaka, B. Molles, N. Sugiyama, P. Marchot, E. Ackermann, S. Malany, J. McArdle, I. Tsigelny & S. M. Sine. Toxins selective for subunit interfaces as probes of nicotinic acetylcholine receptor structure. Journal of Physiology (Paris) 92: 79-84, 1998.
  14. Engel, A. G., K. Ohno & S. M. Sine. Congenital myasthenic syndromes: Experiments of nature. Journal of Physiology (Paris) 92: 113-118, 1998.
  15. Engel, A., K. Ohno, H-L. Wang, M. Milone & S. M. Sine. Molecular basis of congenital myasthenic syndromes: mutations in the acetylcholine receptor. The Neuroscientist 4, 185-194, 1998.
  16. Engel, A. G., K. Ohno & S. M. Sine. Congenital myasthenic syndromes: Recent advances. Archives of Neurology 56, 163-167, 1999.
  17. Engel, A. G., K. Ohno & S. M. Sine. Congenital myasthenic syndromes. In: Engel, A. G. (Ed.): Myasthenia Gravis and Myasthenic Disorders. New York, Oxford University Press, 251-297, 1999.
  18. Engel, A. G, Ohno, K. & Sine, S.M. Acetylcholine receptor channelopathies and other congenital myasthenic syndromes. In: Channelopathies of the nervous system. Rose, M and Griggs, R; editors. Oxford: Butterworth-Heinemann. 179-191, 2001.
  19. Sine, S.M. The nicotinic receptor ligand binding domain. J. Neurobiol. 3, 431-446, 2002.
  20. Engel., A. G., Ohno, K. & Sine, S.M. The spectrum of congenital myasthenic syndromes. Mol. Neurobiol. 26, 347-367, 2002.
  21. Engel., A. G., Ohno, K. & Sine, S.M. Congenital myasthenic syndromes: Progress over the past decade. Muscle and Nerve 27, 4-25, 2003.
  22. Engel, A. G., Ohno, K. & Sine, S.M. Sleuthing molecular targets for neurological diseases: The neuromuscular junction as a paradigm. Nature Reviews Neuroscience 4, 339-352. (2003).
  23. Sine, S.M., Wang, H-L., Ohno, K., Shen, X-M., Lee, W. & Engel, A. G. Mechanistic diversity underlying fast channel congenital myasthenic syndromes. Ann. N. Y. Acad. Sci. 998, 1-10 (2003).
  24. Sine, S.M., A. G. Engel, H.-L. Wang & K. Ohno. Molecular insights into acetylcholine structure and function revealed by mutations causing congenital myasthenic syndromes. In: R. Maue (Ed.): Molecular Insights into Ion Channel Biology in Health and Disease. Elsevier Science (In press).
  25. Sine, S.M., Wang, H-L., & Gao, F. Toward atomic-scale understanding of ligand recognition in the nicotinic receptor. Current Medicinal Chemistry (in press).
  26. Engel., A. G., Ohno, K. & Sine, S.M. Congenital myasthenic syndromes: a diverse array of molecular targets. J. Neurocytology (in press).
  27. Engel., A. G., Ohno, K. & Sine, S.M. Congenital myasthenic syndromes. In: Myology, 3rd Edition (in press).

Abstracts

  1. Sine, S.M., W. Okamura & A. Norman. Solution structure of vitamin D3 studied by high resolution NMR. Pacific Biochemical Slopes Conference, 1974.
  2. Sine, S.M. & P. Taylor. Functional consequences of agonist-induced state transitions in the cholinergic receptor. Federation Proceedings, 1979, 8, p. 273a.
  3. Sine, S.M. & J. H. Steinbach. Agonists block currents through the acetylcholine receptor. Biophysical Journal, 1983, 41, 133a.
  4. Sine, S.M. & J. H. Steinbach. Apparent opening and agonist dissociation rates of the acetylcholine receptor on BC3H-1 cells. Biophysical Journal, 1983, 41, p. 133a.
  5. Sine, S.M. & J. H. Steinbach. Activation of acetylcholine receptors by dimethyl-d-tubocurarine. Biophysical Journal, 1984, 45, p. 386a.
  6. Sine, S.M. & J. H. Steinbach. Activation of acetylcholine receptors by low concentrations of agonist. Biophysical Journal, 1985, 47, p. 42a.
  7. Sine, S.M. & J. H. Steinbach. Activation of acetylcholine receptors by high concentrations of agonist. Biophysical Journal, 1985, 47, p. 42a.
  8. Sine, S.M., T. Claudio & F. Sigworth. Functional properties of Torpedo acetylcholine receptors genetically reconstituted in mouse fibroblasts. Biophysical Journal, 1988, 53, p. 637a.
  9. Sine, S.M., T. Claudio & F. Sigworth. Activation of Torpedo acetylcholine receptors: single channel current kinetics reveal distinct agonist binding affinities. Biophysical Journal, 1990, 57, p.15A.
  10. Kreinkamp, H., S. M. Sine & P. Taylor. Glycosylation interferes with a-toxin binding to the nicotinic receptor. ASBMB, 1993.
  11. Sine, S.M. Molecular dissection of subunit interfaces in the acetylcholine receptor - Identification of residues that determine curare selectivity. Society for Neuroscience, 1993, 19, p. 10.4.
  12. Kreinkamp, H., R. Maeda, S. M. Sine & P. Taylor. Determinants of a-conotoxin specificity for the g and d subunit interfaces of the nicotinic acetylcholine receptor. ASBMB, 1994.
  13. Maeda, R., H. Kreinkamp, S. M. Sine & P. Taylor. Identifying Regions of Interaction Between the Subunits of the Nicotinic Acetylcholine Receptor. ASBMB, 1994.
  14. Fu, D.X. & S. M. Sine. Competitive antagonists bridge the a-g subunit interface of the acetylcholine receptor through quaternary ammonium-aromatic interactions. Biophysical Journal, 1994, 66, p. A9-D1.
  15. Zhang, Y., J. Chen, G. Akk, S. M. Sine & A. Auerbach. Single channel kinetics of mouse AChR:mutations of a-subunit residues Y198, Y190, Y93, and D200. Biophysical Journal, 1994, 66, p. A9-D2.
  16. Prince, R. J. & S. M. Sine. Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that determine agonist selectivity. Biophysical Journal, 1995, 70, p. A251.
  17. Chen, J., Y. Zhang, G. Akk, S. Sine & A. Auerbach. Single-channel kinetics of recombinant mouse AChR: effects of mutation of a subunit residue Y190. Biophysical Journal, 1995, 68, p. A376.
  18. Sine, S., G. Akk & A. Auerbach. Monovalent cations are competitive inhibitors of ACh binding to mouse recombinant nicotinic ACh receptors. Society for Neuroscience, 1995: 21, p. 344.
  19. Zhang, Y., J. Chen, G. Akk, M. Slaughter, S. Sine & A. Auerbach. Residues from non-alpha subunits of nicotinic acetylcholine receptors participate in agonist binding: evidence from single channel studies. Society for Neuroscience, 1995, 21, p. 1582.
  20. Ohno, K., H.-L. Wang, M. Milone, N. Bren, J. M. Brengman, S. Nakano, P. Quiram, J. N. Pruitt, S. M. Sine & E. G. Engel. Congential myasthenic syndrome caused by decreased agonist binding affinity due to a mutation in the acetylcholine receptor e subunit. Biophysical Journal, 1995, 70, p. A372.
  21. Quiram, P. & S. M. Sine. Identification of residues in the d_ subunit that affect cooperative interactions in the nicotinic acetylcholine receptor. Society for Neuroscience, 1996, 22, p. 110.2.
  22. Prince, R. J. & S. M. Sine. Epibatidine selects between the agonist binding sites of muscle nicotinic acetylcholine receptors. Society for Neuroscience, 1996, 22, p. 110.3.
  23. Bren, N. D. & S. M. Sine. Identification of residues that determine curare selectivity in the adult acetylcholine receptor. Society for Neuroscience, 1996, 22, p. 501.4.
  24. Wang, H.-L., N. Bren, A. Auerbach, A. G. Engel & S. M. Sine. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases both the rate of agonist dissociation and rate constants for channel gating. Society for Neuroscience, 1996, 22, p. 501.5.
  25. Milone, M., K. Ohno, J. N. Pruitt, J. M. Brengman, S. M. Sine & A. G. Engel. Congenital myasthenic syndrome (CMS) due to frameshifting acetylcholine receptor (AChR) e subunit mutations. Society for Neuroscience, 1996, 22, p. 763.1.
  26. Prince, R. J. & S. M. Sine. Identification of agonist selectivity determinants in the muscle nicotinic acetylcholine receptor. Biophysical Journal, 1996, 70, A251.
  27. Milone, M., N. Bren, K. Ohno, A. G. Engel & S. M. Sine. Congenital myasthenic syndrome caused by decreased agonist affinity due to mutation in a ligand binding domain of the acetylcholine receptor epsilon subunit. Biophysical Journal, 1996, 70, A372.
  28. Prince, R. &. M. Sine. Determinants of agonist selectivity in the gamma and delta subunits of the nicotinic receptor affect the kinetics of activation. Biophysical Journal, 1997, 72, p. A334.
  29. Quiram, P. A. & S. M. Sine. Pharmacology of the alpha7 neuronal nicotinic receptor: Identification of residues that confer high affinity for antagonists. Society for Neuroscience, 1997, 23, 388.
  30. Ohno, K., P. Quiram, M. Milone, H.-L. Wang, C. M. Harper, J. Pruitt, J. Brengman, L. Pao, K. Fishbeck, T. O. Crawford, S. M. Sine & A. G. Engel. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: Report of 5 new mutations. Neurology, 1997, 48, A73.
  31. Prince, R. J. & S. M. Sine. Kinetics of epibatidine activation of muscle nicotinic receptors. Society for Neuroscience, 1997, 23, 665.
  32. Prince, R. J. & S. M. Sine. Simpkin, a single channel kinetics simulator.or How to do patch clamp without getting yours hands dirty! Society For Neuroscience, 1997, 23, 286.
  33. Zhou, M., J. Chen, G. Akk, S. M. Sine, A. G. Engel & A. Auerbach. Choline as an agonist of wild type and mutant acetylcholine receptor channels. Society for Neuroscience, 1997, 23, 388.
  34. Quiram, P. A. & S. M. Sine. Identification of alpha-conotoxin im1 residues essential for binding to neuronal alpha-7 receptors. Biophysical Journal, 1998, 74, A252.
  35. Zhou, M., F. Salamone, C. Bouzat, S. M. Sine & A. Auerbach. Single-channel characterization of a mouse muscle acetylcholine receptor channel with a mutation at position 433 in the M4 segment of the a subunit. Biophysical Journal, 1998, 74, A90.
  36. Quiram, P. A. & S. M. Sine. Identification of pairs of interacting residues between alpha-conotoxin ImI and neuronal alpha7 receptors. Biophysical Journal, 1999, 76, A22.
  37. Ohno, K., H.L. Wang, X.-M. Shen, M. Milone, L. Bernasconi, S. M. Sine & A. G. Engel. Slow channel mutations in the center of the M1 transmembrane domain of the acetylcholine receptor a _subunit. Neurology (Suppl 3), 1999, 54, A183.
  38. Quiram, P., K. Ohno, M. Milone, M. Patterson, N. Pruitt, J. Brengman, S. M. Sine & A. G. Engel. Acetylcholine receptor b subunit mutation mutations causing endplate AChR deficiency and reduced assembly with the d subunit. Neurology (Suppl 2), 1999, 52, A185.
  39. Bren, N. D. & S. M. Sine. Pairwise interactions between alpha-conotoxin MI and mouse nicotinic acetylcholine receptors. Society for Neuroscience, 1999, 25, 1241.
  40. Wang, H-L. & S. M. Sine. Hidden Markov modeling analysis reveals kinetic diversity of single acetylcholine receptor channel currents. Society for Neuroscience, 1999, 25, 1721.
  41. Bouzat, C, F. Barrantes & S. M. Sine. Mechanistic and structural contributions to channel gating of a conserved threonine in the nicotinic receptor M4 domain. Biophysical Journal, 2000, 78, 359A.
  42. Salamone, F., C. Grosman, S. M. Sine & A. Auerbach. Role of the M2-M3 linker in nicotinic acetylcholine receptor channel gating. Society for Neuroscience. 2000, 26, 137.12.
  43. Prince, R. J., A. H. Weston & S. M. Sine. Tacrine block of muscle nicotinic acetylcholine receptors. British Journal of Pharmacology, 2000, 129-130P.
  44. Prince, R. J., M. Smith, S. E. Fisher & S. M. Sine. Determinants of epibatidine selectivity at desensitised muscle nicotinic acetylcholine receptors. Society for Neuroscience, 2000, 26, 138.16.
  45. Hussain, J. F., D. Lovejoy, D. R. Bristow, S. M. Sine & R. J. Prince. Multiple binding sites for tacrine in the nicotinic acetylcholine receptor channel. Society for Neuroscience, 2000, 26, 526.11.
  46. Lee, W. Y., Bren, N. D. & Sine, S.M. Contribution of branched hydrophobic side chains to acetylcholine binding affinity of the muscle nAChR. Biophysical Journal, 2002, 80, 1040A.
  47. Bouzat, C. B., Gumilar, M., Esandi, C., & Sine, S.M. Subunit-selective contribution to channel gating of the M4 domain of the nicotiniic receptor. Biophysical Journal, 2002, 80, 1042A.
  48. Dilger, J., Vidal, A., Sine, S.M. & Pedersen, S. Kinetics of inhibition of muscle ACh-receptor mutants by curare analogs. Biophysical Journal 2002, 80, 1043A.
  49. Sine, S.M., Wang, H. L., Ohno, K. & Engel, A. G. Nicotinic receptors in health and disease: structure, function and dynamics. Xth International Conference on Myasthenia Gravis and Related Disorders, 2002.

Invited presentations

  1. Gordon Research Conference on Molecular Pharmacology: "Molecular basis of drug action on the nicotinic acetylcholine receptor". Franklin, New Hampshire, June, 1981.
  2. Molecular Pharmacology Conference: "Analysis of drug action on the nicotinic acetylcholine receptor using binding and functional measurements". Guildford, England, August, 1982.
  3. Molecular Biology of Ion Channels: "Function of mammalian nicotinic acetylcholine receptors: Agonist concentration dependence of single channel current kinetics". Yale University, New Haven, CT, November, 1987.
  4. Gordon Research Conference on Ion Channels: "Function of Torpedo acetylcholine receptors expressed in fibroblasts". New London, New Hampshire, August, 1988.
  5. Gordon Research Conference on Ion Channels: "Structure-function relationships in the acetylcholine receptor", June, 1992.
  6. Taniguchi Symposium: "Toward Molecular Biophysics of Ion Channels". Nagoya, Japan, December, 1993.
  7. Ontario and Western New York Ion Channels Meeting: "Structure-function relationships in the acetylcholine receptor: From the ligand binding site to the channel gate". Buffalo, New York, November, 1994.
  8. Gordon Research Conference on Mechanisms of Membrane Transport Proteins: "Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of ligand binding and subunit assembly determinants". Holderness, NH, June, 1995.
  9. Gordon Research Conference on Ion Channels: "Structural and mechanistic bases of mutations causing congenital myasthenic syndromes". Tilton, NH, July, 1996.
  10. International Symposium on Neurobiology IX-Ion Channels and Related Diseases: " Structural and mechanistic bases of mutations causing congenital myasthenic syndromes". Tokyo, Japan, January, 1997.
  11. IXth International Conference on Myasthenia Gravis and Related Disorders: Mechanisms of acetylcholine receptor activation revealed by naturally occurring mutations. Santa Monica, CA, May, 1997.
  12. Xth International Conference on Cholinergic Mechanisms: Molecular dissection of subunit interfaces in the nicotinic acetylcholine receptor. Arcachon, France, September, 1998.
  13. Xth International Conference on Cholinergic Mechanisms: Congenital myasthenic syndromes: experiments of nature. Arcachon, France, September, 1998.
  14. 83rd International Titisee Conference on Ion Channels and Disease: "Mechanistic insights into acetylcholine receptor function revealed by mutations causing congenital myasthenic syndromes". Titisee, Germany, 2001.
  15. Xth International Conference on Myasthenia Gravis and Related Disorders: Nicotinic receptors in health and disease: structure, function and dynamics. Key Biscayne, FL, May, 2002.
  16. NIDA Conference on Neuronal Nicotinic Receptors and Ligands: Toward atomic-scale understanding of ligand recognition in the nicotinic acetylcholine receptor. Bal Harbour, FL, June, 2003.

Invited seminars

  1. The Salk Institute for Biological Studies: "Analysis of drug action on the nicotinic acetylcholine receptor using binding and functional measurements". Host: Dr. Jon Lindstrom, 1980.
  2. SUNY, Buffalo: "Kinetics of acetylcholine receptor activation in clonal mouse muscle cells". Host: Dr. Fred Sachs, 1986.
  3. Cornell University: "Kinetics of acetylcholine receptor activation in clonal mouse muscle cells". Host: Dr. Robert Oswald, 1987.
  4. Rush Medical College: "Activation of Torpedo acetylcholine receptors: Single channel current kinetics reveal distinct agonist binding affinities". Host: Dr. Robert Eisenberg, 1988.
  5. University of California at Santa Barbara: "Activation of Torpedo acetylcholine receptors: Single channel current kinetics reveal distinct agonist binding affinities". Host: Dr. Carol Vandenberg, 1991.
  6. University of California at San Diego: "Gamma and delta subunits regulate the affinity and cooperativity of the acetylcholine receptor". Host: Dr. Palmer Taylor, 1991.
  7. Stanford University: "Gamma and delta subunits regulate the affinity and cooperativity of the acetylcholine receptor". Host: Dr. Richard Tsien, 1991.
  8. University of California at Davis: "Gamma and delta subunits regulate the affinity and cooperativity of the acetylcholine receptor". Host: Dr. Mark McNamee, 1991.
  9. Yale University: "Gamma and delta subunits regulate the affinity and cooperativity of the acetylcholine receptor". Host: Dr. Fred Sigworth, 1991.
  10. Cornell University: "Molecular dissection of subunit interfaces in the acetylcholine receptor". Host: Dr. Greg Weiland, 1992.
  11. Mayo Clinic Rochester: "Identification of residues that determine curare selectivity in the nicotinic acetylcholine receptor". Host: Dr. Tony Windebank, 1993.
  12. University of California at Santa Barbara: "Molecular dissection of subunit interfaces in the acetylcholine receptor". Host: Dr. Carol Vandenberg, 1993.
  13. University of California at San Diego: "Molecular dissection of subunit interfaces in the acetylcholine receptor". Host: Dr. Palmer Taylor, 1993.
  14. SUNY at Buffalo: "Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of ligand binding and gating determinants". Host: Dr. Anthony Auerbach, 1994.
  15. Mayo Clinic at Scottsdale: "Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of ligand binding and gating determinants". Host: Dr. John R. Riordan, 1994.
  16. University of California at San Diego: "Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of ligand binding and gating determinants". Host: Dr. Palmer Taylor, 1994
  17. University of Pittsburgh: "Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that determine conotoxin M1 selectivity". Host: Dr. Stuart Abramson, 1995.
  18. University of California at San Diego: "Molecular dissection of subunit interfaces in the acetylcholine receptor: Identification of residues that stabilize alpha-bungarotoxin binding". Host: Dr. Palmer Taylor, 1997
  19. University of Chicago: Acetylcholine Receptor Structure and Function: insights from a-conotoxins, human mutations and high resolution single channel recording. Host: Dr. William Green, 1999.
  20. Washington University St. Louis: Acetylcholine Receptor Structure and Function: insights from a-conotoxins, human mutations and high resolution single channel recording. Host: Dr. Joe Henry Steinbach, 1999.
  21. University of California at San Diego: Acetylcholine Receptor Structure and Function: insights from a-conotoxins, human mutations and high resolution single channel recording. Host: Dr. Palmer Taylor, 2001.
  22. St. Louis University: Acetylcholine Receptor Structure and Function: insights from a-conotoxins, human mutations and high resolution single channel recording. Host: Dr. Mark Voigt, 2001.
  23. University of Wisconsin: Molecular Biophysics of the Nicotinic Receptor: Insights from mutagenesis, structural modeling and high resolution single channel recording. Host: Dr. Cynthia Czjakowski, 2001.
  24. National Institute of Health: Molecular Biophysics of the Nicotinic Receptor: Insights from mutagenesis, structural modeling and high resolution single channel recording. Host: Dr. Mark Mayer, 2002.

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