HAI-LONG WANG

E-mail: wang.hai-long@mayo.edu

Education:

FuDan University, Shanghai, China
B.S. - Biophysics, 1987

Shanghai Institute of Physiology, Chinese Academia of Science
Ph.D. - Neurobiology, 1994

Professional Experience:

Practice Researcher, Shanghai Institute of Cell Biology, Chinese Academia of Science 7/87 - 7/89

Postdoctoral Research Fellow, Department of Physiology and Biophysics, Mayo Foundation, Rochester MN 1/95 - 1/98

Senior Research Fellow, Department of Physiology and Biophysics,

Mayo Foundation, Rochester MN 1/98 - 1/2001

Research Associate, Department of Physiology and Biophysics,

Mayo Foundation, Rochester MN 1/01-1/2002

Senior Research Associate, Department of Physiology and Biophysics,

Mayo Foundation, Rochester MN 1/02-present

Publications

1. Wang H-L., Wei J-Y and Yang X-L. Suppression of Carp retinal bipolar cells in darkness. Chinese Science Bulletin 39 (2): 158-161, 1994

2. Wang H-L., Wei J-Y., and Yang X-L. Centrifugal fibers to the retina. Sheng Li Ko Hsueh Chin Chan 25, 22-26. 1994. (review)

3. Wei J-Y., Wang H-L., Yang X-L. Effects of acute hypoxia on response of horizontal cells and electroretinograms in carp retina in vivo. Chinese Science Bulletin . 1995

4. Wang H-L., Wei J-Y and Yang X-L. Effect of optic nerve stimulation on bipolar cell in the carp retina. Chinese Science Bulletin 40(5): 422-426, 1995

5. Wei J-Y., Wang H-L., and Yang X-L. Novel GABAc receptors. Sheng Li Ko Hsueh Chin Chan 26, 7-11. 1995. (review)

6. Wang H-L. and Yang X-L. Differential effects of low calcium on signal transmission from rods and cones to horizontal cells in carp retina. Sheng Li Hsueh Pao 48, 113-124. 1996.

7. Ohno, K., H. -L. Wang, M. Milone, N. Bren, J. M. Brengman, S. Nakano, P. Quiram, J.N. Pruitt, S. M. Sine and E. G. Engel. Congential myasthenic syndrome caused by decreased agonist binding affinity due to a mutation in the acetylcholine receptor e subunit. Neuron 17:157-170, 1996.

8. Engel, A. G., K. Ohno, M. Milone, H. -L. Wang, S. Nakano, C. Bouzat, J. N. Pruitt II, D. O. Hutchinson, J. M. Brengman, N. Bren, J. P. Sieb and S. M. Sine: New mutations in acetylcholine receptor subunit genes reveal heterogeneity in the slow-channel congenital myasthenic syndrome. Human Molecular Genetics 5: 1217-1227, 1996.

9. Ohno, K., Quiram, P., Milone, M., Wang, H-L., Harper, M., Pruitt, J., Brengman, J., Pao, L., Fischbeck, K., Crawford, T., Sine, S. and A. Engel. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: identification and functional characterization of six new mutations. Human Molecular Genetics 6: 753-766, 1997.

10. Wang, H-L., Auerbach, A., Bren, N., Ohno, K., Engel, A., and S. Sine. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases the rate of agonist dissociation. Journal of General Physiology 109: 757-766, 1997.

11. Milone, M., Wang, H-L., Ohno, K., Fukudome, T., Pruitt, N., Bren, N., Sine, S., and A. Engel. Slow channel myasthenic syndrome caused by enhanced activation, desensitization, and agonist binding affinity due to mutation in the M2 domain of the acetylcholine receptor a subunit. Journal of Neuroscience 17: 5651-5665, 1997.

12. Wang, H-L., Milone, M., Ohno, K., Prince, R., Fukudome, T., Shen, X-M., Brengman, J., Griggs, R., Sine, S. M., and A. Engel. Mode switching kinetics by a naturally occurring mutation in the cytoplasmic loop of the human acetylcholine receptor e subunit. Neuron 20: 575-588, 1998.

13. Engel, A., K. Ohno, H-L. Wang, M. Milone and S. M. Sine. Molecular basis of congenital myasthenic syndromes: mutations in the acetylcholine receptor. The Neuroscientist 4, 185-194, 1998

14. Wang, H.-L., Milone, M., Ohno, K., Shen, X.-M., Tsujuno, A., Paola, A., Tonali, P., Brengman, J., Engel, A. G. and Sine, S. M. Acetylcholine receptor M3 domain: stereochemical and volume contributions to channel gating. Nature Neuroscience 2, 226-233, 1999.

15. Wang, H.-L, Ohno, K., Milone, M., Brengman, J. Evoli, A., Batocchi, A. P., Middleton, L., Christodoulou, K., Engel, A. G. and Sine, S. M. Fundamental gating mechanism of nicotinic receptor channel gating revealed by mutation causing a congenital myasthenic syndrome. J. Gen. Physiol., 116, 449-460, 2000.

16. Iba¯ez-Tallon, I., Miwa, J., Wang, H.L., Adams, N., Crabtree, G., Sine, S. M. and Heintz, N. Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1. Neuron 33,893-903,2002.

17. Sine, S. M., Engel, A. G., Wang, H.-L., and Ohno, K. Molecular insights into acetylcholine structure and function revealed by mutations causing congenital myasthenic syndromes. In: R. Maue (Ed.) Molecular Insights into Ion Channel Biology in Health and Disease. Elsevier Science (In press).

18. Sine, S. M., Shen, X. M., Wang, H. L., Brengmann, J., Lee, W. Y. Bren, N. and Engel, A. G. State-specific contributions to agonist affinity revealed by naturally-occurring mutations in the human acetylcholine receptor epsilon subunit (In press JGP).

19. Sine. S. M., Wang, H.-L. and Bren, N. Lysine scanning mutagenesis delineates structure of nicotinic receptor ligand binding domain (In press JBC).

Abstracts:

1. Ohno, K., Wang, H.-L., Milone, M., Bren, N., Brengman, J. M., Nakano, S., Quiram, P., Pruitt, J. N., Sine, S. M. and Engel, E. G. Congential myasthenic syndrome caused by decreased agonist-binding affinity due to a mutation in the acetylcholine receptor e subunit. Biophysical Journal, 1995, 70, p. A372.

2. Wang, H.-L., Bren, N., Auerbach, A., Engel, A. G.and Sine, S. M. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases both the rate of agonist dissociation and rate constants for channel gating. Society for Neuroscience, 1996, 22, p. 501.5.

3. Ohno, K., Quiram, P., Milone, M., Wang, H-L., Harper, C. M., Pruitt, J., Brengman, J., Pao, L., Fishbeck, K., Crawford, T. O., Sine, S. M. and Engel, A. G. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: Report of 5 new mutations. Neurology, 1997, 48, A73.

4. Ohno, K., Wang, H-L., Shen, X-M. Milone, M., Bernasconi, L., Sine, S. M. and Engel, A. G. Slow channel mutations in the center of the M1 transmembrane domain of the acetylcholine receptor a subunit. Neurology (Suppl 3), 1999, 54, A183.

5. Wang, H-L. and Sine, S. M. Hidden Markov modeling analysis reveals kinetic diversity of single acetylcholine receptor channel currents. Society for Neuroscience, 1999, 25, 1721.

6. Fan Gao, Hai-Long Wang, Steven M. Sine. Dynamic simulation and ligand docking study of Acetylcholine receptor ligand binding domain.

Professional Societies:

Society for Neuroscience

Grant Awards:

Fellowship from Myasthenia Gravis Foundation 1998.