Methods

Xenopus oocyte preparation

for a detailed protocol of oocyte methods and expression cloning, see:

1. Romero, MF, Y Kanai, H Gunshin, MA Hediger. Expression cloning using Xenopus laevis oocytes. Meth Enz. 296: 17-52, 1998.

Microelectrode preparation:

1. pH and Na ⁺ microelectrodes:
   Sciortino CM, and MF Romero. Cation and voltage dependence of the rat kidney, electrogenic Na⁺/HCO^–₃ cotransporter, rkNBC, expressed in oocytes. Am. J. Physiol., 277: 611-623, 1999.
2. pH, Cl^- and Na⁺ microelectrodes:
   Romero MF, D Henry, S Nelson, PJ Harte, AK Dillon and CM Sciortino. Cloning and characterization of a Na⁺ driven anion exchanger (NDAE1): a new bicarbonate transporter. J. Biol. Chem. 275: 24552-9, 2000.

Immunohistochemistry:

1. Sciortino, CM, LD Shrode, BR Fletcher, PJ Harte, MF Romero. Localization of Endogenous and Recombinant Na⁺-driven Anion Exchanger Protein, NDAE1, from Drosophila melanogaster. Am. J. Physiol. Cell Physiol. 281: C449-463, 2001.